Reviewed by: Nick Nicotra, Chief Science Officer
Research References

Methylene Blue Clinical Trials Database

Structured database of methylene blue clinical trials registered on ClinicalTrials.gov. Browse by condition, phase, and status. Links to published results where available. For the underlying science behind these trials, see our clinical evidence overview.

12Registered Trials
11Completed
9Conditions Studied
5Phase III

Filter by Condition

Cognitive Function (1)Alzheimer's Disease (3)Vasoplegic Syndrome (1)Septic Shock (1)Malaria (2)Depression (1)Anxiety / PTSD (1)Stroke (1)Encephalopathy (1)

All Registered Trials

NCT IDConditionPhaseStatusYearOutcome
NCT01626391[PMC]Cognitive FunctionPhase IICompleted2016Improved fMRI BOLD signal during attention tasks (p<0.05)
NCT01689246Alzheimer's DiseasePhase IIICompleted2016Primary endpoints not met; secondary signals in monotherapy
NCT01689233Alzheimer's DiseasePhase IIICompleted2016Primary endpoints not met
NCT00515333EncephalopathyPhase IICompleted2010Prevention of ifosfamide neurotoxicity
NCT01365390Vasoplegic SyndromePhase IIICompleted2015Reduced vasopressor requirements
NCT00473616MalariaPhase IICompleted2009Parasite clearance rates
NCT02380573Septic ShockPhase IICompleted2018Hemodynamic stability outcomes
NCT03446001DepressionPhase IICompleted2020Hamilton Depression Rating Scale changes
NCT02594930MalariaPhase IIICompleted2017Fever clearance and parasite reduction
NCT04370288Alzheimer's DiseasePhase IIICompleted2022Long-term safety and efficacy monitoring
NCT02819193[PMC]Anxiety / PTSDPhase ICompleted2018Fear extinction retention
NCT01253122StrokePhase IITerminated2014Study terminated early

Research Landscape by Condition

Cognitive Enhancement

The Rodriguez et al. (2016) study (NCT01626391) remains the highest-quality evidence for cognitive effects, using fMRI to demonstrate improved attention and memory retrieval.

View Brain Health Research →

Alzheimer's Disease

Multiple Phase III trials of LMTM (TauRx) did not meet primary endpoints. Secondary analyses showed mixed signals. This is an honest acknowledgment of current trial status.

View Alzheimer's Research →

Critical Care (Vasoplegic / Sepsis)

IV methylene blue has established clinical use for vasoplegic syndrome following cardiac surgery and septic shock. These represent the most successful clinical applications, with reduced vasopressor requirements documented.

Malaria

Multiple trials in endemic regions have studied MB as combination therapy. Results show antimalarial activity, though this represents a different use case than supplement-context research.

Understanding Clinical Trial Data

Database Methodology

This database includes trials registered on ClinicalTrials.gov with methylene blue as an intervention. Selection prioritizes trials with published results or completed status. IV administration trials (critical care) are included for completeness but represent different use cases than oral supplementation. For a transparent discussion of what this evidence supports and where gaps remain, see our balanced analysis of MB evidence. Last updated: March 2026.

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